Publications

  1. Oberstaller J, Xu S, Naskar D, Zhang M, Wang C, Gibbons J, Pires CV, Mayho M, Otto TD, Rayner JC, Adams JH: Supersaturation mutagenesis reveals adaptive rewiring of essential genes among malaria parasites. Science 2025, 387:eadq7347.

  2. Dass S, Kundu P, Naskar D, Kanjee U, Ferreira MU, Rayner JC, Duraisingh MT: A Miniaturized Assay to Evaluate Plasmodium cynomolgi and Plasmodium knowlesi as Models for Prioritizing Plasmodium vivax Vaccine Targets. The Journal of Infectious Diseases 2025:jiaf136.

  3. Kariuki SN, Gilchrist JJ, Uyoga S, Macharia A, Makale J, Rayner JC, Williams TN: Relation Between the Dantu Blood Group Variant and Bacteremia in Kenyan Children: A Population-Based Case-Control Study. The Journal of Infectious Diseases 2025, 231:e10-e16.

  4. Pires CV, Chawla J, Sollelis L, Oberstaller J, Zhang M, Wang C, Gibbons J, Rayner JC, Otto TD, Marti M: Genetic factors regulating Plasmodium falciparum gametocytogenesis identified by phenotypic screens. Scientific Reports 2024, 14:31010.

  5. Kioko M, Mwangi S, Pance A, Ochola-Oyier LI, Kariuki S, Newton C, Bejon P, Rayner JC, Abdi AI: The mRNA content of plasma extracellular vesicles provides a window into molecular processes in the brain during cerebral malaria. Science Advances 2024, 10:eadl2256.

  6. Frischknecht F, Rayner JC, Waters AP: 20 years of BioMalPar: Building a collaborative malaria research network. Trends in Parasitology 2024.

  7. Rayner J: Relation between the Dantu blood group variant and bacteraemia in Kenyan children: a population-based case control study. 2024.

  8. Kals E, Kals M, Cicuta P, Rayner JC: Under conditions of high wall shear stress, several PfEBA and PfRH ligands are important for Plasmodium falciparum malaria blood-stage growth. bioRxiv 2024:2024.2009. 2024.614720.

  9. Kebede AM, Sutanto E, Trimarsanto H, Benavente ED, Barnes M, Pearson RD, Siegel SV, Erko B, Assefa A, Getachew S, et al: Genomic analysis of Plasmodium vivax describes patterns of connectivity and putative drivers of adaptation in Ethiopia. Sci Rep 2023, 13:20788.

  10. Kioko M, Pance A, Mwangi S, Goulding D, Kemp A, Rono M, Ochola-Oyier LI, Bull PC, Bejon P, Rayner JC: Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum. Nature Communications 2023, 14:6447.

  11. Kariuki SN, Macharia AW, Makale J, Nyamu W, Hoffman SL, Kapulu MC, Bejon P, Rayner JC, Williams TN: The Dantu blood group prevents parasite growth in vivo: evidence from a controlled human malaria infection study. Elife 2023, 12:e83874.

  12. Simmons CF, Gibbons J, Zhang M, Oberstaller J, Pires CV, Casandra D, Wang C, Seyfang A, Otto TD, Rayner JC: Protein KIC5 is a novel regulator of artemisinin stress response in the malaria parasite Plasmodium falciparum. Scientific Reports 2023, 13:399.

  13. Pires CV, Oberstaller J, Wang C, Casandra D, Zhang M, Chawla J, Adapa SR, Otto TD, Ferdig MT, Rayner JC: Chemogenomic profiling of a Plasmodium falciparum transposon mutant library reveals shared effects of dihydroartemisinin and bortezomib on lipid metabolism and exported proteins. Microbiology spectrum 2023, 11:e05014-05022.

  14. Kengne-Ouafo JA, Bah SY, Kemp A, Stewart L, Amenga-Etego L, Deitsch KW, Rayner JC, Billker O, Binka FN, Sutherland CJ: The global transcriptome of Plasmodium falciparum mid-stage gametocytes (stages II–IV) appears largely conserved and gametocyte-specific gene expression patterns vary in clinical isolates. Microbiology Spectrum 2023, 11:e03820-03822.

  15. Abdi A, Mwikali K, Mwangi S, Pance A, Ochola-Oyier L, Kariuki S, Newton C, Bejon P, Rayner J: The mRNA content of plasma extracellular vesicles provides a window into the brain during cerebral malaria disease progression. Science Advances 2023:1-17.

  16. Levashina E, Soldati‐Favre D, Waters AP, Frischknecht F, Rayner JC: Malaria: moving beyond the search for magic bullets. EMBO Molecular Medicine 2023, 15:e18727.

  17. Pance A, Ng BL, Mwikali K, Koutsourakis M, Agu C, Rouhani FJ, Montandon R, Law F, Ponstingl H, Rayner JC: Novel stem cell technologies are powerful tools to understand the impact of human factors on Plasmodium falciparum malaria. Frontiers in Cellular and Infection Microbiology 2023, 13:1287355.

  18. Carrasquilla M, Drammeh NF, Rawat M, Sanderson T, Zenonos Z, Rayner JC, Lee MC: Barcoding genetically distinct Plasmodium falciparum strains for comparative assessment of fitness and antimalarial drug resistance. Mbio 2022, 13:e00937-00922.

  19. Introini V, Govendir MA, Rayner JC, Cicuta P, Bernabeu M: Biophysical tools and concepts enable understanding of asexual blood stage malaria. Frontiers in Cellular and Infection Microbiology 2022, 12:908241.

  20. Kariuki SN, Macharia AW, Makale J, Nyamu W, Hoffman SL, Kapulu MC, Bejon P, Rayner JC, Williams TN, Team C-SS: Controlled Human Malaria Infection reveals that the Dantu blood group variant provides high level protection against uncomplicated malaria. medRxiv 2022:2022.2009. 2021.22280031.

  21. Ndegwa DN, Kundu P, Hostetler JB, Marin-Menendez A, Sanderson T, Mwikali K, Verzier LH, Coyle R, Adjalley S, Rayner JC: Using Plasmodium knowlesi as a model for screening Plasmodium vivax blood-stage malaria vaccine targets reveals new candidates. PLOS Pathogens 2021, 17:e1008864.

  22. Oberstaller J, Otto TD, Rayner JC, Adams JH: Essential genes of the parasitic apicomplexa. Trends in parasitology 2021, 37:304-316.

  23. Ndwiga L, Osoti V, Ochwedo KO, Wamae K, Bejon P, Rayner JC, Githinji G, Ochola-Oyier LI: The Plasmodium falciparum Rh5 invasion protein complex reveals an excess of rare variant mutations. Malaria Journal 2021, 20:278.

  24. Pance A, Ling B, Mwikali K, Koutsourakis M, Agu C, Rouhani F, Ponstingl H, Montandon R, Law F, Rayner JC: Stem cell technology provides novel tools to understand human variation in Plasmodium falciparum MALARIA. BioRxiv 2021:2021.2006. 2030.450498.

  25. Knöckel J, Dundas K, Yang AS, Galaway F, Metcalf T, Van Gemert G-J, Sauerwein RW, Rayner JC, Billker O, Wright GJ: Systematic identification of Plasmodium falciparum sporozoite membrane protein interactions reveals an essential role for the p24 complex in host infection. Molecular & Cellular Proteomics 2021, 20:100038.

  26. Chappell L, Ross P, Orchard L, Russell TJ, Otto TD, Berriman M, Rayner JC, Llinás M: Refining the transcriptome of the human malaria parasite Plasmodium falciparum using amplification-free RNA-seq. BMC genomics 2020, 21:1-19.

  27. Siegel SV, Chappell L, Hostetler JB, Amaratunga C, Suon S, Böhme U, Berriman M, Fairhurst RM, Rayner JC: Analysis of Plasmodium vivax schizont transcriptomes from field isolates reveals heterogeneity of expression of genes involved in host-parasite interactions. Scientific Reports 2020, 10:16667.

  28. Carrasquilla M, Adjalley S, Sanderson T, Marin-Menendez A, Coyle R, Montandon R, Rayner JC, Pance A, Lee MC: Defining multiplicity of vector uptake in transfected Plasmodium parasites. Scientific Reports 2020, 10:10894.

  29. Siegel SV, Rayner JC: Single cell sequencing shines a light on malaria parasite relatedness in complex infections. Trends in Parasitology 2020, 36:83-85.

  30. Mwangi SJ, Gwela A, Kioko M, Bargul JL, Nduati EW, Ndungu FM, Bejon P, Rayner JC, Abdi AI: Impact of Plasmodium falciparum small-sized extracellular vesicles on host peripheral blood mononuclear cells [version 1; peer review: awaiting peer review. 2020.

  31. Knöcke J, Dundas K, Yang AS, Galaway F, Metcalf T, van Gemert G-J, Sauerwein RW, Rayner JC, Billker O, Wright GJ: Systematic identification of P. falciparum sporozoite membrane protein interactions reveals an essential role for the p24 complex in host infection. Molecular and Cellular Proteomics 2020.

  32. Carrasquilla M, Sanderson T, Montandon R, Rayner J, Pance A, Lee M: Quantitation of vector uptake reveals non-Poissonian transfection dynamics in Plasmodium falciparum. bioRxiv 2019:681981.

  33. Howick VM, Russell AJ, Andrews T, Heaton H, Reid AJ, Natarajan K, Butungi H, Metcalf T, Verzier LH, Rayner JC: The Malaria Cell Atlas: Single parasite transcriptomes across the complete Plasmodium life cycle. Science 2019, 365:eaaw2619.

  34. Hillier C, Pardo M, Yu L, Bushell E, Sanderson T, Metcalf T, Herd C, Anar B, Rayner JC, Billker O: Landscape of the Plasmodium interactome reveals both conserved and species-specific functionality. Cell reports 2019, 28:1635-1647. e1635.

  35. Lo E, Hostetler JB, Yewhalaw D, Pearson RD, Hamid MM, Gunalan K, Kepple D, Ford A, Janies DA, Rayner JC: Frequent expansion of Plasmodium vivax Duffy Binding Protein in Ethiopia and its epidemiological significance. PLoS neglected tropical diseases 2019, 13:e0007222.

  36. Verzier LH, Coyle R, Singh S, Sanderson T, Rayner JC: Plasmodium knowlesi as a model system for characterising Plasmodium vivax drug resistance candidate genes. PLoS neglected tropical diseases 2019, 13:e0007470.

  37. Hillier C, Pardo Calvo M, Yu L, Bushell E, Sanderson T, Metcalf T, Herd C, Anar B, Rayner JC, Billker O: Landscape of the Plasmodium Interactome. 2019.

  38. Draper SJ, Sack BK, King CR, Nielsen CM, Rayner JC, Higgins MK, Long CA, Seder RA: Malaria Vaccines: Recent Advances and New Horizons. Cell Host Microbe 2018, 24:43-56.

  39. Draper SJ, Sack BK, King CR, Nielsen CM, Rayner JC, Higgins MK, Long CA, Seder RA: Malaria vaccines: recent advances and new horizons. Cell host & microbe 2018, 24:43-56.

  40. Claessens A, Harris LM, Stanojcic S, Chappell L, Stanton A, Kuk N, Veneziano-Broccia P, Sterkers Y, Rayner JC, Merrick CJ: RecQ helicases in the malaria parasite Plasmodium falciparum affect genome stability, gene expression patterns and DNA replication dynamics. PLoS genetics 2018, 14:e1007490.

  41. Tarr SJ, Díaz-Ingelmo O, Stewart LB, Hocking SE, Murray L, Duffy CW, Otto TD, Chappell L, Rayner JC, Awandare GA: Schizont transcriptome variation among clinical isolates and laboratory-adapted clones of the malaria parasite Plasmodium falciparum. BMC genomics 2018, 19:1-13.

  42. Theron M, Cross N, Cawkill P, Bustamante LY, Rayner JC: An in vitro erythrocyte preference assay reveals that Plasmodium falciparum parasites prefer Type O over Type A erythrocytes. Scientific reports 2018, 8:8133.

  43. Tarr SJ, Díaz-Ingelmo O, Stewart LB, Hocking SE, Murray L, Duffy CW, Otto TD, Chappell L, Rayner JC, Awandare GA: Plasmodium falciparum schizont stage transcriptome variation among clinical isolates and laboratory-adapted clones. bioRxiv 2018:329532.

  44. Hamilton WL, Claessens A, Otto TD, Kekre M, Fairhurst RM, Rayner JC, Kwiatkowski D: Extreme mutation bias and high AT content in Plasmodium falciparum. Nucleic Acids Res 2017, 45:1889-1901.

  45. Chiappino Pepe A, Bushell E, Limenitakis R, Rayner JC, Heussler V, Billker O, Hatzimanikatis V: Identifying and targeting key cellular mechanisms for proliferation in Plasmodium parasites: a combined experimental and computational strategy. In 3rd International SystemsX ch Conference on Systems Biology2017

  46. Chiappino Pepe A, Bushell E, Rayner JC, Billker O, Hatzimanikatis V: Toward the identification of cellular mechanisms behind the lethal phenotypes in malaria parasites blood stages with PlasmoGEM and metabolic modeling. In Biochemical and Molecular Engineering XX2017

  47. Modrzynska K, Pfander C, Chappell L, Yu L, Suarez C, Dundas K, Gomes AR, Goulding D, Rayner JC, Choudhary J: A knockout screen of ApiAP2 genes reveals networks of interacting transcriptional regulators controlling the Plasmodium life cycle. Cell host & microbe 2017, 21:11-22.

  48. Hamilton WL, Claessens A, Otto TD, Kekre M, Fairhurst RM, Rayner JC, Kwiatkowski D: Extreme mutation bias and high AT content in Plasmodium falciparum. Nucleic acids research 2017, 45:1889-1901.

  49. Galaway F, Drought LG, Fala M, Cross N, Kemp AC, Rayner JC, Wright GJ: P113 is a merozoite surface protein that binds the N terminus of Plasmodium falciparum RH5. Nature communications 2017, 8:14333.

  50. Tham W-H, Beeson JG, Rayner JC: Plasmodium vivax vaccine research–we’ve only just begun. International journal for parasitology 2017, 47:111-118.

  51. Abdi A, Yu L, Goulding D, Rono MK, Bejon P, Choudhary J, Rayner J: Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyan clinical isolate defines a core parasite secretome. Wellcome open research 2017, 2:50.

  52. Sanderson T, Rayner JC: PhenoPlasm: a database of disruption phenotypes for malaria parasite genes. Wellcome open research 2017, 2:45.

  53. Sanderson T, Rayner JC: PlasmoTron: an open-source platform for automated culture of malaria parasites. BioRxiv 2017:241596.

  54. Bronner IF, Otto TD, Zhang M, Udenze K, Wang C, Quail MA, Jiang RH, Adams JH, Rayner JC: Quantitative insertion-site sequencing (QIseq) for high throughput phenotyping of transposon mutants. Genome research 2016, 26:980-989.

  55. Hopp CS, Balaban AE, Bushell ES, Billker O, Rayner JC, Sinnis P: Palmitoyl transferases have critical roles in the development of mosquito and liver stages of Plasmodium. Cellular microbiology 2016, 18:1625-1641.

  56. Tay CL, Jones ML, Hodson N, Theron M, Choudhary JS, Rayner JC: Study of Plasmodium falciparum DHHC palmitoyl transferases identifies a role for PfDHHC9 in gametocytogenesis. Cellular microbiology 2016, 18:1596-1610.

  57. Yiangou L, Montandon R, Modrzynska K, Rosen B, Bushell W, Hale C, Billker O, Rayner JC, Pance A: A stem cell strategy identifies glycophorin C as a major erythrocyte receptor for the rodent malaria parasite Plasmodium berghei. PLoS One 2016, 11:e0158238.

  58. Ahouidi AD, Amambua-Ngwa A, Awandare GA, Bei AK, Conway DJ, Diakite M, Duraisingh MT, Rayner JC, Zenonos ZA: Malaria vaccine development: focusing field erythrocyte invasion studies on phenotypic diversity: the West African Merozoite Invasion Network (WAMIN). Trends in parasitology 2016, 32:274-283.

  59. Billker O, Rayner JC: Calcium Builds Strong Host-Parasite Interactions. Cell Host & Microbe 2015, 18:9-10.

  60. Weiss GE, Gilson PR, Taechalertpaisarn T, Tham W-H, de Jong NW, Harvey KL, Fowkes FJ, Barlow PN, Rayner JC, Wright GJ: Revealing the sequence and resulting cellular morphology of receptor-ligand interactions during Plasmodium falciparum invasion of erythrocytes. PLoS pathogens 2015, 11:e1004670.

  61. Gomes AR, Bushell E, Schwach F, Girling G, Anar B, Quail MA, Herd C, Pfander C, Modrzynska K, Rayner JC: A genome-scale vector resource enables high-throughput reverse genetic screening in a malaria parasite. Cell host & microbe 2015, 17:404-413.

  62. Doerig C, Rayner JC, Scherf A, Tobin AB: Post-translational protein modifications in malaria parasites. Nature Reviews Microbiology 2015, 13:160-172.

  63. Schwach F, Bushell E, Gomes AR, Anar B, Girling G, Herd C, Rayner JC, Billker O: Plasmo GEM, a database supporting a community resource for large-scale experimental genetics in malaria parasites. Nucleic acids research 2015, 43:D1176-D1182.

  64. Zenonos ZA, Dummler SK, Müller-Sienerth N, Chen J, Preiser PR, Rayner JC, Wright GJ: Basigin is a druggable target for host-oriented antimalarial interventions. Journal of Experimental Medicine 2015, 212:1145-1151.

  65. Pinheiro MM, Ahmed MA, Millar SB, Sanderson T, Otto TD, Lu WC, Krishna S, Rayner JC, Cox-Singh J: Plasmodium knowlesi genome sequences from clinical isolates reveal extensive genomic dimorphism. PLoS One 2015, 10:e0121303.

  66. Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, Rayner JC: A library of Plasmodium vivax recombinant merozoite proteins reveals new vaccine candidates and protein-protein interactions. PLoS neglected tropical diseases 2015, 9:e0004264.

  67. Rayner JC: Plasmodium malariae malaria: from monkey to man? EBioMedicine 2015, 2:1023-1024.

  68. Hodson N, Invergo B, Rayner JC, Choudhary JS: Palmitoylation and palmitoyl-transferases in Plasmodium parasites. Biochemical Society Transactions 2015, 43:240-245.